Biotech Lab Protocols, Tips & Tricks

For 1M ,1000ml Tris 121.14 gm is needed;

Therefore, for 0.7M ,1000ml, xgm is needed;

Therefore x= 0.7*121.14=84.728 gm;

Now for 0.7M ,70 ml, Tris needed is x gm

X =70*84.728/1000=5.93 gm

Therefore 5.93 gm of Tris is required to make 0.7 M ,70 ml stock

2) 0.5M, 20ml EDTA:

...

372.2gm of EDTA in 1000 ml=1M

Therefore 186.12 of EDTA in 1000ml=0.5M

X gm in 20ml =0.5 M

Therefore x =186.1*0.5/1000 =3.7224gm

3) NaOH:

40GM NaOH in 1000 ml = in

X gm of NaOH in 50 ml=1N; =40*50/1000=2gm

4) 5M, 5O ML OF SODIUM ACETATE:

83.03gm in 1000ml =1 M

415.15gm in 1000 ml=5M

X gm in 50 ml=5M therefore x= 50*415.5/1000 =20.75 gm

5) AMPICILLIN:

For solid media 100µg/ml

For liquid media 50µg /ml

6) SDS (1%,100ml):

1% means 1gm SDS in 100ml distilled water.

7) TBE BUFFER:

In 1000 ml, Tris base is 54gm , boric acid is 27.5gm, 0.5 EDTA is 20 ml, For 100 ml

Tris base =54*100/1000=5.4gm

Boric acid=27.5*100/1000=2.75gm

EDTA =20*100/1000=2mL

8) ETHANOL:

absolute ethanol = 100ml

70% ethanol = 100ml