Biotech Lab Protocols, Tips & Tricks

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1) TENS BUFFER: 300µl *5=1500µl+backup 200µl = 1700µl

2) 3M SODIUM ACETATE: 150µl*5=750µl+backup250µl = 1000µl

3) 100% ETHANOL: 0.9ml*5 =4.5ml+backup 3.0ml = 7.5ml

4) 70% ETHANOL: 2ml*5 =10ml+backup 5 ml = 15ml

5) DISTILLED WATER: 300µl*5=150µl+backup 50µl = 200µl

6) OVERNIGHT BACTERIAL CULTURE: 1.5ml*5=7.5ml+backup 2.5ml = 10ml

7) AMPICILLIN:

FOR LIQUID MEDIA: C1*V1=C2*V2; 50000* v1=100*100000; V1=200µL

FOR SOLID MEDIA:/strong> 50000*V1=50*100000, V1=100µL

E:For 50 ml, 5*v1=.5*50=5ml

For 500ml, 5*v1=.5*500=50ml