Tags: e.coli
PLASMID DNA ISOLATION FROM E.coli (TENS Method) FlowChart
The flow chart below describes the procedure of isolating Plasmid DNA from E. coli. more »
PLASMID DNA ISOLATION FROM E.coli (TENS Method)
PURPOSE: To isolate plasmid DNA from E.coli for further uses like restriction digestion & quantification experiments.INSTRUMENTS: Autoclave, Centrifuge, Refrigerator,Shaker incubator, Micro pippetts and tips, appendorf tubes.PRINCIPLE: The isolation… more »
Culturing Strains for Plasmid Isolation
AIM: To culture strain-DH5a of E.coli for isolation of plasmid DNA.MATERIALS REQUIRED:1.LBA media - 100ml2.Glycerol stock of DH5a3.Ampicillin 50ul4.Inoculation loop5.Burner6.Petri dishINTRODUCTION: Characteristics of E.coli(DH5a):It transforms with hig… more »
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