Tags: ethanol
Preparation of Reagents required for Stock Preparation
1) 0.7M ,70ml Tris:For 1M ,1000ml Tris 121.14 gm is needed; Therefore, for 0.7M ,1000ml, xgm is needed; Therefore x= 0.7*121.14=84.728 gm;
Now for 0.7M ,70 ml, Tris needed is x gmX =70*84.728/1000=5.93 gmTherefore 5.93 gm of Tris is required to ma… more »
Preparation of Reagents required for Plasmid Isolation
PURPOSE:
To prepare the reagents required for the plasmid isolation.NAME STOCK CONC. WORKING CONC. Tris(pH 7.5) 0.7M 10mM EDTA(pH 8) 0.5M 1mM NaOH 1N 0.1N SDS 1.00% 0.50% Sodium Acetate(pH5.2) 5M 3M Ampicillin Liquid:100ug/ml
Solid:50ug/ml Liquid:20… more »
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